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Using MapMan

Gene Level vs Transcript Level?

Gene Level vs Transcript Level?
Answer
10/25/16 7:08 PM
Hi! I'm a new user of MapMan, and am confused about the mapping files. I did RNAseq on some samples, and did gene-level counting. I used the phytozome Citrus genome, and it looks like the mapping file available for MapMan is for the transcripts only. I did a 'quick and dirty' analysis with MapMan using one of the transcripts as a representative for each of the genes that I found to be differentially expressed (e.g. if Gene A contains transcripts 1, 2, and 3, then I jused used Transcript1 as a proxy for GeneA so I can see something on the pathway maps), but I don't think this is the best way to do analysis. Is there a gene-level map available? When I try to make my own, I get a lot of redundancies, so some genes show up on pathways multiple times.